Molecular-Scale
Investigations Reveal
Noncovalent Bonding Underlying the Adsorption of Environmental DNA
on Mica
Posted on 2019-09-11 - 12:11
Mineral–soil
organic matter (SOM including DNA, proteins, and polysaccharides)
associations formed through various interactions, play a key role
in regulating long-term SOM preservation. The mechanisms underlying
DNA–mineral and DNA–protein/polysaccharide interactions
at nanometer and molecular scales in environmentally relevant solutions
remain uncertain. Here, we present a model mineral–SOM system
consisting of mineral (mica)–nucleic acid (environmental DNA,
eDNA)/protein (bovine serum albumin)/polysaccharide (alginate), and
combine atomic force microscopy (AFM)-based dynamic force spectroscopy
and PeakForce quantitative nanomechanical mapping using DNA-decorated
tips. Single-molecule binding and adhesion force of eDNA to mineral
and to mineral adsorbed by protein/polysaccharide reveal the noncovalent
bonds and that systematically changing ion compositions, ionic strength,
and pH result in significant differences in organic–organic
and organic–mineral binding energies. Consistent with the bond-strength
measurements, protein, rather than polysaccharide, promotes mineral-bound
DNA molecules by ex situ AFM deposition observations in relatively
high concentrations of divalent cation-containing acidic solutions.
These molecular-scale determinations and nanoscale observations should
substantially improve our understanding of how environmental factors
influence the organic–mineral interfacial interactions through
the synergy of collective noncovalent and/or covalent bonds in mineral–organic
associations.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Zhai, Hang; Wang, Lijun; Putnis, Christine V. (2019). Molecular-Scale
Investigations Reveal
Noncovalent Bonding Underlying the Adsorption of Environmental DNA
on Mica. ACS Publications. Collection. https://doi.org/10.1021/acs.est.9b04064
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (3)
HZ
Hang Zhai
LW
Lijun Wang
CP
Christine V. Putnis
KEYWORDS
bond-strength measurementsmolecular-scale determinationsmineral-bound DNA moleculesfactors influenceinteractiondivalent cation-containing acidic solutionsSOM preservationEnvironmental DNADNA-decorated tipspH resultion compositionsnanoscale observationsadhesion forceproteinnanomechanical mappingforce microscopyMolecular-Scale Investigations Reveal Noncovalent BondingSingle-molecule bindingAFM deposition observationsnoncovalent bondsforce spectroscopy