Minimally Invasive Multimode Optical Fiber
Microendoscope for Deep Brain Fluorescence
Imaging
Posted on 2018-03-06 - 13:50
A major open challenge in Neuroscience is the ability to
measure and perturb neural activity in vivo from well defined
neural sub-populations at cellular resolution
anywhere in the brain. However, limitations posed by
scattering and absorption prohibit non-invasive
multi-photon approaches for deep (>2mm) structures,
while Gradient Refractive Index (GRIN) endoscopes are
relatively thick and can cause significant damage upon insertion. Here, we
present a novel micro-endoscope design to image neural
activity at arbitrary depths via an ultra-thin multi-mode
optical fiber (MMF) probe that has 5-10X thinner diameter than
commercially available micro-endoscopes. We demonstrate
micron-scale resolution, multi-spectral and volumetric
imaging. In contrast to previous approaches, we show
that this method has an improved acquisition speed that is
sufficient to capture rapid neuronal dynamics in-vivo in
rodents expressing a genetically encoded calcium indicator (GCaMP).
Our results emphasize the potential of this technology in
Neuroscience applications and open up possibilities for
cellular resolution imaging in previously unreachable brain
regions.
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Ohayon, Shay; Aguirre, Antonio Miguel Caravaca; Piestun, Rafael; DiCarlo, James (2018). Minimally Invasive Multimode Optical Fiber
Microendoscope for Deep Brain Fluorescence
Imaging. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3968625.v1
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AUTHORS (4)
SO
Shay Ohayon
AA
Antonio Miguel Caravaca Aguirre
RP
Rafael Piestun
JD
James DiCarlo