Mechanical Properties of Giant Liposomes Compressed
between Two Parallel Plates: Impact of Artificial Actin Shells
Posted on 2013-08-20 - 00:00
The
mechanical response of giant liposomes to compression between
two parallel plates is investigated in the context of an artificial
actin cortex adjacent to the inner leaflet of the bilayer. We found
that nonlinear membrane theory neglecting the impact of bending sufficiently
describes the mechanical response of liposomes consisting of fluid
lipids to compression whereas the formation of an actin cortex or
the use of gel-phase lipids generally leads to substantial stiffening
of the shell. Giant vesicles are gently adsorbed on glassy surfaces
and are compressed with tipless cantilevers using an atomic force
microscope. Force–compression curves display a nonlinear response
that allows us to determine the membrane tension σ0 and the area compressibility modulus KA by computing the contour of the vesicle as a function of the compression
depth. The values for KA of fluid membranes
correspond well to what is known from micropipet-suction experiments
and computed from monitoring membrane undulations. The presence of
a thick actin shell adjacent to the inner leaflet of the liposome
membrane stiffens the system considerably, as mirrored in a significantly
higher apparent area compressibility modulus.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Schäfer, Edith; Kliesch, Torben-Tobias; Janshoff, Andreas (2016). Mechanical Properties of Giant Liposomes Compressed
between Two Parallel Plates: Impact of Artificial Actin Shells. ACS Publications. Collection. https://doi.org/10.1021/la401969t
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (3)
ES
Edith Schäfer
TK
Torben-Tobias Kliesch
AJ
Andreas Janshoff