Large-Scale 3D Optical Mapping and Quantitative Analysis
of Nanoparticle Distribution in Tumor Vascular Microenvironment
Posted on 2020-06-15 - 20:14
Nanoparticles
(NPs) are a promising carrier for cancer therapeutics.
Systemically administered NPs are transported to tumor tissues via
the bloodstream, extravasated from microvessels, and delivered to
cancer cells. The distribution of NPs in the tumor vascular microenvironment
critically determines the therapeutic efficacy of NP-delivered drugs,
but its precise assessment in 3D across a large volume remains challenging.
Here, an analytical platformtermed OMNIA (for Optical Mapping
of Nanoparticles and Image Analysis)integrating tissue clearing,
high-resolution optical imaging, and semiautomated image analysis
is presented, which enables accurate, unbiased, and quantitative analysis
of the distribution of NPs in relation to the vasculature across a
large 3D volume. Application of OMNIA to tumor tissues revealed higher
accumulation and more efficient extravasation of NPs in the tumor
periphery than the core. Time-course analysis demonstrated that the
accumulation of NPs in tumor peaked at 24 h after injection, but the
relative distribution of NPs from the vasculature remained remarkably
stable over time. Comparisons between 45- and 200-nm-sized NPs showed
a lower accumulation of smaller NPs in tumors relative to the liver,
yet better vessel permeation. Together, our results demonstrate that
OMNIA facilitates precise and reliable evaluation of NP biodistribution,
and mechanistic investigations on NP delivery to tumor tissues.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Koo, Dong-Jun; Choi, Jinahn; Ahn, Minchul; Ahn, Benjamin H.; Min, Dal-Hee; Kim, Sung-Yon (2020). Large-Scale 3D Optical Mapping and Quantitative Analysis
of Nanoparticle Distribution in Tumor Vascular Microenvironment. ACS Publications. Collection. https://doi.org/10.1021/acs.bioconjchem.0c00263
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (6)
DK
Dong-Jun Koo
JC
Jinahn Choi
MA
Minchul Ahn
BA
Benjamin H. Ahn
DM
Dal-Hee Min
SK
Sung-Yon Kim