Label-free multi-photon imaging using a compact femtosecond fiber laser mode-locked by carbon nanotube saturable absorber
Posted on 2017-04-26 - 16:45
We demonstrate label-free multi-photon imaging of biological samples using a compact Er3+-doped femtosecond fiber laser mode-locked by a single-walled carbon nanotube (CNT). These compact and low cost lasers have been developed by various groups but they have not been exploited for multiphoton microscopy. Here, it is shown that various multiphoton imaging modalities (e.g. second harmonic generation (SHG), third harmonic generation (THG), two-photon excitation fluorescence (TPEF), and three-photon excitation fluorescence (3PEF)) can be effectively performed on various biological samples using a compact handheld CNT mode-locked femtosecond fiber laser operating in the telecommunication window near 1560nm. We also show for the first time that chlorophyll fluorescence in plant leaves and diatoms can be observed using 1560nm laser excitation via three-photon absorption.
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Kieu, K.; Mehravar, S.; Gowda, R.; Norwood, R. A.; Peyghambarian, N. (2013). Label-free multi-photon imaging using a compact femtosecond fiber laser mode-locked by carbon nanotube saturable absorber. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3752411.v1
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R. A. Norwood
telecommunication windowCNT mode-locked femtosecond fiber laserTPEFgenerationcost laserssampleTHGPEF1560 nmthree-photon absorptionsingle-walled carbon nanotubemultiphoton microscopymultiphoton imaging modalitieschlorophyll fluorescencecarbon nanotube saturable absorberSHGLabel-free multi-photon imagingfemtosecond fiber laser mode-locked1560 nm laser excitationlabel-free multi-photon imaging