E. coli Nissle 1917 Affects Salmonella Adhesion to Porcine Intestinal Epithelial Cells

H. Wieler, Lothar

E. coli Nissle 1917 Affects Salmonella Adhesion to Porcine Intestinal Epithelial Cells

Posted on 2013-02-20 - 20:25

Confluent monolayers of IPEC-J2 cells were pre-incubated with E. coli Nissle 1917 (EcN), E. coli 140815 or E. coli MG1655 at an MOI of 100∶1 or 10∶1 bacteria to host cells. After two hours, cells were washed and infected with Salmonella Typhimurium using an MOI of 100∶1 Salmonella to host cells. Invasion levels in percent (%) are expressed as invasion of Salmonella relative to invasion without pre-incubation with E. coli (Salmonella mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to Salmonella mono-infection.

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Schierack, Peter; Kleta, Sylvia; Tedin, Karsten; Tachu Babila, Julius; Oswald, Sibylle; A. Oelschlaeger, Tobias; et al. (2015). E. coli Nissle 1917 Affects Salmonella Adhesion to Porcine Intestinal Epithelial Cells. PLOS ONE. Collection. https://doi.org/10.1371/journal.pone.0014712

https://doi.org/10.1371/journal.pone.0014712

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