Hilbert-Huang single-shot spatially multiplexed interferometric microscopy

H2S2MIM (initials incoming from Hilbert-Huang Single-shot Spatially Multiplexed Interferometric Microscopy) is presented as the implementation of a robust, fast and accurate single-shot phase estimation algorithm with an extremely simple, low cost and highly stable way to convert a bright field microscope into a holographic one using partially coherent illumination. All together, H2S2MIM adds high-speed (video frame rate) quantitative phase imaging capability to a commercially available non-holographic microscope with improved phase reconstruction (coherence noise reduction). The technique has been validated using a 20X/0.46NA objective in a regular Olympus BX-60 upright microscope for static as well as dynamic samples showing perfect agreement with the results retrieved from temporal phase shifting algorithm.