Ganoderterpene A, a New Triterpenoid from Ganoderma
lucidum, Attenuates LPS-Induced Inflammation
and Apoptosis via Suppressing MAPK and TLR-4/NF-κB Pathways
in BV‑2 Cells
Posted on 2021-10-20 - 07:29
An investigation of the fruiting
bodies of edible mushroom Ganoderma lucidum produced 13 steroids, containing
one new lanostane-type triterpene compound, named ganoderterpene A
(1). Nuclear magnetic resonance and high-resolution electrospray
ionization mass spectrometry data were used to deduce these structures.
All the isolates were evaluated for their ability to suppress NO generation
in BV-2 microglial cells treated with lipopolysaccharide (LPS) and
exhibited moderate to strong inhibition effects, with IC50 values in the range 7.15–36.88 μM.
Among the tested compounds, compound 1 exhibited the
most marked activity with an IC50 value of 7.15 μM, and the structure–activity relationships
were studied. This study showed that compound 1 significantly
suppressed the activation of MAPK and TLR-4/NF-κB signaling
pathways, as evidenced by an immunofluorescence assay and a molecular
docking experiment. Furthermore, compound 1 effectively
improved the LPS-induced mitochondrial membrane potential and apoptosis.
These findings suggest that ganoderterpene A could exert protective
effects in microglial cells from apoptosis by restraining the inflammatory
response. Hence, G. lucidum could be
used as a novel preventative agent for neurodegenerative disorders.
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Kou, Rong-Wei; Gao, Yu-Qi; Xia, Bing; Wang, Jia-Yun; Liu, Xiao-Ning; Tang, Jiang-Jiang; et al. (1753). Ganoderterpene A, a New Triterpenoid from Ganoderma
lucidum, Attenuates LPS-Induced Inflammation
and Apoptosis via Suppressing MAPK and TLR-4/NF-κB Pathways
in BV‑2 Cells. ACS Publications. Collection. https://doi.org/10.1021/acs.jafc.1c04905
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AUTHORS (8)
RK
Rong-Wei Kou
YG
Yu-Qi Gao
BX
Bing Xia
JW
Jia-Yun Wang
XL
Xiao-Ning Liu
JT
Jiang-Jiang Tang
XY
Xia Yin
JG
Jin-Ming Gao
KEYWORDS
strong inhibition effectsnuclear magnetic resonancenovel preventative agentmolecular docking experimentκb signaling pathwaystype triterpene compound15 – 3650 sub1 bκb pathwaysμ tested compoundsstudy showedsignificantly suppressednew triterpenoidneurodegenerative disordersmicroglial cellsmarked activitylucidum couldinflammatory responseinduced inflammationimmunofluorescence assayganoderma lucidumfruiting bodiesfindings suggesteffectively improved(< b