Fluorescent ZnS Quantum Dots–Phosphoethanolamine
Nanoconjugates for Bioimaging Live Cells in Cancer Research
Version 2 2018-11-16, 18:05
Version 1 2018-11-16, 18:03
Posted on 2018-11-16 - 18:05
Many
human diseases, including metabolic, immune, and central nervous
system disorders, as well as several types of cancers, are the consequence
of an important alteration in lipid-related metabolic biomolecules.
Although recognized that one of the most important metabolic hallmarks
of cancer cells is deregulation of lipid metabolism, the multiple
complex signaling pathways are poorly understood yet. Thus, in this
research, novel nanoconjugates made of ZnS quantum dots (QDs) were
directly synthesized in aqueous media using phosphoethanolamine (PEA)
as the capping ligand, which is an important biomolecule naturally
present in cells for de novo biosynthesis of fatty acids and phospholipids
involved in the cell structure (e.g., membrane), differentiation,
and cancer growth. These QD–PEA bio-nanoconjugates were characterized
by spectroscopical and morphological techniques. The results demonstrated
that fluorescent ZnS nanocrystalline QDs were produced with uniform
spherical morphology and estimated sizes of 3.3 ± 0.6 nm. These
nanoconjugates indicated core–shell colloidal nanostructures
(ZnS QD–PEA) with the hydrodynamic diameter (HD) of 26.0 ± 3.5 nm and ζ-potential centered
at −30.0 ± 4.5 mV. The cell viability response using mitochondrial
activity assay in vitroconfirmed no cytotoxicity at several concentrations
of PEA (biomolecule) and the ZnS–PEA nanoconjugates. Moreover,
these nanoconjugates effectively behaved as fluorescent nanomarkers
for tracking the endocytic pathways of cancer cells using confocal
laser scanning microscopy bioimaging. Hence, these results proved
that biofunctionalized ZnS–PEA nanoprobes offer prospective
tools for cellular bioimaging with encouraging forecast for future
applications as active fluorescent biomarker conjugates in metabolic-related
cancer research.
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Mansur, Alexandra
A. P.; Mansur, Herman S.; Carvalho, Sandhra M.; Lobato, Zélia I.
P.; Leite, Maria de Fátima; Mansur, Lorena L. (2018). Fluorescent ZnS Quantum Dots–Phosphoethanolamine
Nanoconjugates for Bioimaging Live Cells in Cancer Research. ACS Publications. Collection. https://doi.org/10.1021/acsomega.8b02098
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AUTHORS (6)
AM
Alexandra
A. P. Mansur
HM
Herman S. Mansur
SC
Sandhra M. Carvalho
ZL
Zélia I.
P. Lobato
ML
Maria de Fátima Leite
LM
Lorena L. Mansur