Fiber-based 920 nm femtosecond laser for two-photon microscopy

Fevrier, Sebastien

Fiber-based 920 nm femtosecond laser for two-photon microscopy

Posted on 2018-10-11 - 13:41

We introduce a fiber-based laser system providing 130 fs pulses with 3.5 nJ energy at 920 nm at a 43 MHz repetition rate and illustrate the potential of the source for two-photon excited fluorescence microscopy of living mouse brain. The laser source is based on frequency-doubling high-energy solitons generated and frequency-shifted to 1840 nm in large mode area fibers. This simple laser system could unleash the potential of two-photon microscopy techniques in the biology laboratory where green fluorescent proteins with emission spectrum peaking around 920 nm are used in routine.

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Hage, Charles-Henri; Gomes, Jean-Thomas; Bardet, Sylvia; Granger, Geoffroy; Jossent, Mathieu; Lavoute, Laure; et al. (2018). Fiber-based 920 nm femtosecond laser for two-photon microscopy. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4172732.v1

https://doi.org/10.6084/m9.figshare.c.4172732.v1

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