Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Posted on 2018-05-29 - 05:00
Abstract Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling Aâ˘T to Gâ˘C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants. An endogenous gene is also successfully modified through introducing a gain-of-function point mutation to directly produce an herbicide-tolerant rice plant. With this new adenine base editing system, it is now possible to precisely edit all base pairs, thus expanding the toolset for precise editing in plants.
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Li, Chao; Zong, Yuan; Wang, Yanpeng; Jin, Shuai; Zhang, Dingbo; Song, Qianna; et al. (2018). Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4114730.v1
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AUTHORS (8)
CL
Chao Li
YZ
Yuan Zong
YW
Yanpeng Wang
SJ
Shuai Jin
DZ
Dingbo Zhang
QS
Qianna Song
RZ
Rui Zhang
CG
Caixia Gao