Interferon-λ₄ (IFNL4) Transcript Expression in Human Liver Tissue Samples

After completion of PCR amplification, 1 µl of samples was separated and analyzed in the Agilent 2100 bioanalyzer using the DNA 1000 LabChip kit. Electropherograms and gels are shown for two representative samples each of ss469415590 TT homozygotes without any amplification of IFNL4-specific transcripts (IFNL4 nd) (#1, #2) or of ΔG homozygotes (#3, #4) and of TT homozygotes (#5, #6) with detectable amounts of hepatic IFNL4-specific mRNA (IFNL4 d). Analysis confirmed the lack of products in samples #1 and #2, and the presence of products of the expected size (accuracy ±5 bp, as given by the supplier) both in ΔG and TT homozygotes.