Elasto-Inertial Focusing of Mammalian Cells and Bacteria
Using Low Molecular, Low Viscosity PEO Solutions
Version 2 2017-10-18, 18:33
Version 1 2017-10-18, 14:26
Posted on 2017-10-18 - 18:33
The
ability to manipulate biological cells is critical in a diversity
of biomedical and industrial applications. Microfluidic-based cell
manipulations provide unique opportunities for sophisticated and high-throughput
biological assays such as cell sorting, rare cell detection, and imaging
flow cytometry. In this respect, cell focusing is an extremely useful
functional operation preceding downstream biological analysis, since
it allows the accurate lateral and axial positioning of cells moving
through microfluidic channels, and thus enables sophisticated cell
manipulations in a passive manner. Herein, we explore the utility
of viscoelastic carrier fluids for enhanced elasto-inertial focusing
of biological species within straight, rectangular cross section microfluidic
channels. Since the investigated polymer solutions possess viscosities
close to that of water and exhibit negligible shear thinning, focusing
occurs over a wide range of elasticity numbers and a large range of
Reynolds numbers. With a view to applications in the robust focusing
of cells and bacteria, we assess and characterize the influence of
accessible focusing parameters, including blockage ratio, volumetric
flow rate, cell concentration, and polymer chain length.
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Holzner, Gregor; Stavrakis, Stavros; deMello, Andrew (2017). Elasto-Inertial Focusing of Mammalian Cells and Bacteria
Using Low Molecular, Low Viscosity PEO Solutions. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.7b03093
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AUTHORS (3)
GH
Gregor Holzner
SS
Stavros Stavrakis
Ad
Andrew deMello
KEYWORDS
Microfluidic-based cell manipulationscell manipulationsReynolds numbersLow Molecularflow rateelasticity numberscarrier fluidspolymer solutionsimaging flow cytometrysection microfluidic channelsmicrofluidic channelscell detectioncell concentrationblockage ratiopolymer chain lengthLow Viscosity PEO SolutionsMammalian Cells