Effects
of Nanoprobe Morphology on Cellular Binding
and Inflammatory Responses: Hyaluronan-Conjugated Magnetic Nanoworms
for Magnetic Resonance Imaging of Atherosclerotic Plaques
Version 2 2018-03-27, 19:33
Version 1 2018-03-26, 19:46
Posted on 2018-03-27 - 19:33
Atherosclerosis
is an inflammatory disease of arterial walls and
the rupturing of atherosclerotic plaques is a major cause of heart
attack and stroke. Imaging techniques that can enable the detection
of atherosclerotic plaques before clinical manifestation are urgently
needed. Magnetic resonance imaging (MRI) is a powerful technique to
image the morphology of atherosclerotic plaques. In order to better
analyze molecular processes in plaques, contrast agents that can selectively
bind to plaque receptors will prove invaluable. CD44 is a cell surface
protein overexpressed in plaque tissues, the level of which can be
correlated with the risks of plaque rupture. Thus, targeting CD44
is an attractive strategy for detection of atherosclerotic plaques.
Herein, we report the synthesis of hyaluronan-conjugated iron oxide
nanoworms (HA-NWs). A new purification and gel electrophoresis protocol
was developed to ensure the complete removal of free HA from HA-NWs.
Compared to the more traditional spherical HA-bearing nanoparticles,
HA-NWs had an elongated shape, which interacted much stronger with
CD44-expressing cells in CD44- and HA-dependent manners. Furthermore,
the HA-NWs did not induce much inflammatory response compared to the
spherical HA nanoparticles. When assessed in vivo, HA-NWs enabled
successful imaging of atherosclerotic plaques in a clinically relevant
model of ApoE knockout transgenic mice for noninvasive plaque detection
by MRI. Thus, nanoprobe shape engineering can be a useful strategy
to significantly enhance their desired biological properties.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Hossaini Nasr, Seyedmehdi; Tonson, Anne; El-Dakdouki, Mohammad H.; Zhu, David C.; Agnew, Dalen; Wiseman, Robert; et al. (2018). Effects
of Nanoprobe Morphology on Cellular Binding
and Inflammatory Responses: Hyaluronan-Conjugated Magnetic Nanoworms
for Magnetic Resonance Imaging of Atherosclerotic Plaques. ACS Publications. Collection. https://doi.org/10.1021/acsami.7b19708
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
AUTHORS (8)
SH
Seyedmehdi Hossaini Nasr
AT
Anne Tonson
ME
Mohammad H. El-Dakdouki
DZ
David C. Zhu
DA
Dalen Agnew
RW
Robert Wiseman
CQ
Chunqi Qian
XH
Xuefei Huang
KEYWORDS
MRIgel electrophoresis protocolMagnetic resonance imagingHA-NWnoninvasive plaque detectionatherosclerotic plaquesnanoprobe shape engineeringAtherosclerotic Plaques AtherosclerosisHACD 44CD 44-expressing cellsMagnetic Resonance Imaginghyaluronan-conjugated iron oxide nanowormscell surface protein overexpressed