ECM Mechano-Sensing Regulates Cytoskeleton Assembly
and Receptor-Mediated Endocytosis of Nanoparticles
Version 2 2017-06-22, 19:05
Version 1 2017-06-22, 14:51
Posted on 2017-06-22 - 19:05
It is possible to
create sophisticated and target-specific devices
for nanomedicine thanks to technological advances in the engineering
of nanomaterials. When on target, these nanocarriers often have to
be internalized by cells in order to accomplish their diagnostic or
therapeutic function. Therefore, the control of such uptake mechanism
by active targeting strategy has today become the new challenge in
nanoparticle designing. It is also well-known that cells are able
to sense and respond to the local physical environment and that the
substrate stiffness, and not only the nanoparticle design, influences
the cellular internalization mechanisms. In this frame, our work reports
on the cyclic relationship among substrate stiffness, cell cytoskeleton
assembly and internalization mechanism. Nanoparticles uptake has been
investigated in terms of the mechanics of cell environment, the resulting
cytoskeleton activity and the opportunity of activate molecular specific
molecular pathways during the internalization process. To this aim,
the surface of 100 nm polystyrene nanoparticles was decorated with
a tripeptide (RGD and a scrambled version as a control), which was
able to activate an internalization pathway directly correlated to
the dynamics of the cell cytoskeleton, in turn, directly correlated
to the elastic modulus of the substrates. We found that the substrate
stiffness modulates the uptake of nanoparticles by regulating structural
parameters of bEnd.3 cells as spreading, volume, focal adhesion, and
mechanics. In fact, the nanoparticles were internalized in larger
amounts both when decorated with RGD, which activated an internalization
pathway directly correlated to the cell cytoskeleton, and when cells
resided on stiffer material that, in turn, promoted the formation
of a more structured cytoskeleton. This evidence indicates the directive
role of the mechanical environment on cellular uptake of nanoparticles,
contributing new insights to the rational design and development of
novel nanocarrier systems.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Panzetta, Valeria; Guarnieri, Daniela; Paciello, Antonio; Della Sala, Francesca; Muscetti, Ornella; Raiola, Luca; et al. (2017). ECM Mechano-Sensing Regulates Cytoskeleton Assembly
and Receptor-Mediated Endocytosis of Nanoparticles. ACS Publications. Collection. https://doi.org/10.1021/acsbiomaterials.7b00018
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (8)
VP
Valeria Panzetta
DG
Daniela Guarnieri
AP
Antonio Paciello
FD
Francesca Della Sala
OM
Ornella Muscetti
LR
Luca Raiola
PN
Paolo Netti
SF
Sabato Fusco