Dynamic and Functional Profiling of Xylan-Degrading
Enzymes in Aspergillus Secretomes Using Activity-Based
Probes
Version 3 2019-06-26, 07:34
Version 2 2019-05-24, 20:31
Version 1 2019-05-24, 20:29
Posted on 2019-06-26 - 07:34
Plant polysaccharides
represent a virtually unlimited feedstock
for the generation of biofuels and other commodities. However, the
extraordinary recalcitrance of plant polysaccharides toward breakdown
necessitates a continued search for enzymes that degrade these materials
efficiently under defined conditions. Activity-based protein profiling
provides a route for the functional discovery of such enzymes in complex
mixtures and under industrially relevant conditions. Here, we show
the detection and identification of β-xylosidases and endo-β-1,4-xylanases in the secretomes of Aspergillus niger, by the use of chemical probes inspired
by the β-glucosidase inhibitor cyclophellitol. Furthermore,
we demonstrate the use of these activity-based probes (ABPs) to assess
enzyme–substrate specificities, thermal stabilities, and other
biotechnologically relevant parameters. Our experiments highlight
the utility of ABPs as promising tools for the discovery of relevant
enzymes useful for biomass breakdown.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Schröder, Sybrin
P.; de Boer, Casper; McGregor, Nicholas G. S.; Rowland, Rhianna J.; Moroz, Olga; Blagova, Elena; et al. (2019). Dynamic and Functional Profiling of Xylan-Degrading
Enzymes in Aspergillus Secretomes Using Activity-Based
Probes. ACS Publications. Collection. https://doi.org/10.1021/acscentsci.9b00221
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (25)
SS
Sybrin
P. Schröder
Cd
Casper de Boer
NM
Nicholas G. S. McGregor
RR
Rhianna J. Rowland
OM
Olga Moroz
EB
Elena Blagova
JR
Jos Reijngoud
MA
Mark Arentshorst
DO
David Osborn
MM
Marc D. Morant
EA
Eric Abbate
MS
Mary A. Stringer
KK
Kristian B. R. M. Krogh
LR
Lluís Raich
CR
Carme Rovira
JB
Jean-Guy Berrin
GP
Gilles P. van Wezel
AF
Arthur F. J. Ram
BF
Bogdan I. Florea
GA
Gijsbert A. van der Marel