Diurnal cycling of rhizosphere bacterial communities is associated with shifts in carbon metabolism
Posted on 2017-06-24 - 05:00
Abstract Background The circadian clock regulates plant metabolic functions and is an important component in plant health and productivity. Rhizosphere bacteria play critical roles in plant growth, health, and development and are shaped primarily by soil communities. Using Illumina next-generation sequencing and high-resolution mass spectrometry, we characterized bacterial communities of wild-type (Col-0) Arabidopsis thaliana and an acyclic line (OX34) ectopically expressing the circadian clock-associated cca1 transcription factor, relative to a soil control, to determine how cycling dynamics affected the microbial community. Microbial communities associated with Brachypodium distachyon (BD21) were also evaluated. Results Significantly different bacterial community structures (P = 0.031) were observed in the rhizosphere of wild-type plants between light and dark cycle samples. Furthermore, 13% of the community showed cycling, with abundances of several families, including Burkholderiaceae, Rhodospirillaceae, Planctomycetaceae, and Gaiellaceae, exhibiting fluctuation in abundances relative to the light cycle. However, limited-to-no cycling was observed in the acyclic CCAox34 line or in soil controls. Significant cycling was also observed, to a lesser extent, in Brachypodium. Functional gene inference revealed that genes involved in carbohydrate metabolism were likely more abundant in near-dawn, dark samples. Additionally, the composition of organic matter in the rhizosphere showed a significant variation between dark and light cycles. Conclusions The results of this study suggest that the rhizosphere bacterial community is regulated, to some extent, by the circadian clock and is likely influenced by, and exerts influences, on plant metabolism and productivity. The timing of bacterial cycling in relation to that of Arabidopsis further suggests that diurnal dynamics influence plant-microbe carbon metabolism and exchange. Equally important, our results suggest that previous studies done without relevance to time of day may need to be reevaluated with regard to the impact of diurnal cycles on the rhizosphere microbial community.
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Staley, Christopher; Ferrieri, Abigail; Tfaily, Malak; Cui, Yaya; Chu, Rosalie; Wang, Ping; et al. (2017). Diurnal cycling of rhizosphere bacterial communities is associated with shifts in carbon metabolism. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.3810292.v1
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AUTHORS (20)
CS
Christopher Staley
AF
Abigail Ferrieri
MT
Malak Tfaily
YC
Yaya Cui
RC
Rosalie Chu
PW
Ping Wang
JS
Jared Shaw
CA
Charles Ansong
HB
Heather Brewer
AN
Angela Norbeck
MM
Meng Markillie
Fd
Fernanda do Amaral
TT
Thalita Tuleski
TP
Tomás Pellizzaro
BA
Beverly Agtuca
RF
Richard Ferrieri
ST
Susannah Tringe
LP
Ljiljana Paša-Tolić
GS
Gary Stacey
MS
Michael Sadowsky