Defensive Function of Transposable Elements in Bacteria
Version 2 2019-08-19, 15:40
Version 1 2019-08-16, 21:03
Posted on 2019-08-19 - 15:40
It
has been widely debated whether transposable elements have a
positive or a negative effect on their host cells. This study demonstrated
that transposable elements, specifically insertion sequences (ISs),
can adopt a defensive role in Escherichia coli. In
three different E. coli strains (S17, DH5α,
and Nissle 1917), IS1 and IS10 rapidly disrupted the I-CeuI gene (encoding I-CeuI endonuclease) on the plasmid pLO11-ICeuI as
early as the first generation, despite the gene-circuit being under
control of an arabinose promoter. Proteomics analysis showed that
the protein abundance profile of E. coli DH5α
with pLO11-ICeuI in the fifth generation was nearly opposite to that
of control strain (E. coli with pLO11, no I-CeuI).
The DNA damage caused by the leaky expression of I-CeuI was enough to trigger a SOS response and alter lipid synthesis,
ribosomal activity, RNA/DNA metabolism, central dogma and cell cycle
processes in E. coli DH5α. After the ISs
disrupted the expression of I-CeuI, cells fully recovered
by the 31st generation had a protein abundance profile similar to
that of the control strain. This study showed that ISs readily mutated
a harmful gene which subsequently restored host fitness. These observations
have implications for the stability of designed gene circuits in synthetic
biology.
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Fan, Catherine; Wu, Yin-Hu; Decker, Christoph M.; Rohani, Reza; Gesell Salazar, Manuela; Ye, Hua; et al. (2019). Defensive Function of Transposable Elements in Bacteria. ACS Publications. Collection. https://doi.org/10.1021/acssynbio.9b00218
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AUTHORS (9)
CF
Catherine Fan
YW
Yin-Hu Wu
CD
Christoph M. Decker
RR
Reza Rohani
MG
Manuela Gesell Salazar
HY
Hua Ye
ZC
Zhanfeng Cui
FS
Frank Schmidt
WH
Wei E. Huang