DNA Origami Nanopillars
as Standards for Three-Dimensional
Superresolution Microscopy
Posted on 2013-02-13 - 00:00
Nanopillars are promising nanostructures composed of
various materials
that bring new functionalities for applications ranging from photovoltaics
to analytics. We developed DNA nanopillars with a height of 220 nm
and a diameter of ∼14 nm using the DNA origami technique. Modifying
the base of the nanopillars with biotins allowed selective, upright,
and rigid immobilization on solid substrates. With the help of site-selective
dye labels, we visualized the structure and determined the orientation
of the nanopillars by three-dimensional fluorescence superresolution
microscopy. Because of their rigidity and nanometer-precise addressability,
DNA origami nanopillars qualify as scaffold for the assembly of plasmonic
devices as well as for three-dimensional superresolution standards.
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Schmied, Jürgen
J.; Forthmann, Carsten; Pibiri, Enrico; Lalkens, Birka; Nickels, Philipp; Liedl, Tim; et al. (2016). DNA Origami Nanopillars
as Standards for Three-Dimensional
Superresolution Microscopy. ACS Publications. Collection. https://doi.org/10.1021/nl304492y
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AUTHORS (7)
JS
Jürgen
J. Schmied
CF
Carsten Forthmann
EP
Enrico Pibiri
BL
Birka Lalkens
PN
Philipp Nickels
TL
Tim Liedl
PT
Philip Tinnefeld