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Single Chain Variable Fragments Produced in Escherichia coli against Heat-Labile and Heat-Stable Toxins from Enterotoxigenic E. coli

Posted on 2015-07-08 - 03:23

(A) scFv-LT amino acids sequence: VH—Heavy chain variable domain, L—Linker and VL—Light chain variable domain sequence. CDRs are highlighted in yellow for VH and in blue for VL. (B) SDS-PAGE analysis of scFv-LT recombinant antibody expression and purification. Lanes: 1. C43(DE3) non-induced fraction; 2. C43(DE3) induced fraction; 3. Insoluble fraction; 4. Purified scFv-LT.

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AUTHORS (13)

Christiane Y. Ozaki
Caio R. F. Silveira
Fernanda B. Andrade
Roberto Nepomuceno
Anderson Silva
Danielle D. Munhoz
Bruno B. Yamamoto
Daniela Luz
Patrícia A. E. Abreu
Denise S. P. Q. Horton
Waldir P. Elias
Oscar H. P. Ramos
Roxane M. F. Piazza
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