Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum
Posted on 2015-12-09 - 05:00
Abstract A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal genus Thermofilum; Thermofilum pendens was its closest valid relative (95.7 % of sequence identity). Strain 1807-2 grew organothrophically using polysaccharides (starch and glucomannan), yeast extract or peptone as substrates. The addition of other crenarchaea culture broth filtrates was obligatory required for growth and could not be replaced by the addition of these organisms’ cell wall fractions, as it was described for T. pendens. The genome of strain 1807-2 was sequenced using Illumina and PGM technologies. The average nucleotide identities between genome of strain 1807-2 and T. pendens strain HRK 5T and “T. adornatus” strain 1910b were 85 and 82 %, respectively. On the basis of 16S rRNA gene sequence phylogeny, ANI calculations and phenotypic differences we propose a novel species Thermofilum uzonense with the type strain 1807-2T (= DSM 28062T = JCM 19810T). Project information and genome sequence was deposited in Genbank under IDs PRJNA262459 and CP009961, respectively.
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Toshchakov, Stepan; Korzhenkov, Aleksei; Samarov, Nazar; Mazunin, Ilia; Mozhey, Oleg; Shmyr, Ilya; et al. (2016). Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.3608933.v1
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AUTHORS (13)
ST
Stepan Toshchakov
AK
Aleksei Korzhenkov
NS
Nazar Samarov
IM
Ilia Mazunin
OM
Oleg Mozhey
IS
Ilya Shmyr
KD
Ksenia Derbikova
ET
Evgeny Taranov
ID
Irina Dominova
EB
Elizaveta Bonch-Osmolovskaya
MP
Maxim Patrushev
OP
Olga Podosokorskaya
IK
Ilya Kublanov