Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft
Posted on 2016-07-13 - 05:00
Abstract Background Cancer therapies that kill cancer cells without affecting normal cells is the ultimate mode of treating cancers. The VP3, an avian virus-derived protein, can specifically initiate cell death through several signal transduction pathways leading to apoptosis. In cancer, chemoresistance and cell survivability implicate the cell surface protein, CD147. Methods In this study, transfection of VP3 and silencing of CD147 genes was achieved through the treatment of tumors with pVIVO1-GFP/VP3 (VP3), psiRNA-CD147/2 (shCD147/2), and their combination of CT26 colon cancer cell-induced in mice. The effectiveness of tumor-treatment was ascertained by electrophoresis, TUNEL assay, and flow cytometry analysis. While histopathological and biochemical analysis were used as toxic side effect identification. Results The tumor growth delay index (TGDI) after treatment with VP3, shCD147/2, and their combination treatments increased by 1.3-, 1.2-, 2.0- and 2.3-fold respectively, over untreated control. The VP3-shCD147/2 combination treatment was more efficacious then either VP3 or shCD147/2 alone in the retardation of mouse CT26 colorectal cell tumor allograft. Conclusion The antitumor effect of the combination treatment is the result of synergistic effects of VP3 and shCD147/2 on the tumor cells resulting in apoptosis. Thus, the study shows that combination of VP3 and shCD147/2 treatment can be developed into a potential approach for anticolorectal cancer treatment regimen.
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Ismail, Ruzila; Allaudin, Zeenathul; Abdullah, Rasedee; Mohd Lila, Mohd-Azmi; Nik Abd. Rahman, Nik-Mohd-Afizan; Abdul Rahman, Sheikh-Omar (2016). Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.3628277.v1
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AUTHORS (6)
RI
Ruzila Ismail
ZA
Zeenathul Allaudin
RA
Rasedee Abdullah
MM
Mohd-Azmi Mohd Lila
NN
Nik-Mohd-Afizan Nik Abd. Rahman
SA
Sheikh-Omar Abdul Rahman