Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam
Posted on 2017-04-26 - 17:45
Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.
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Xu, Dongli; Zhou, Weibin; Peng, Leilei (2017). Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3753500.v1
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resolution imaging3 D lifetime imagingdual-color Bessel beamimaging depthBessel FmFLIM tomography systemtomography systemimage capability25 μ mFmFLIM systemexcitation-emission channelsdual-color Bessel beam Fourier multiplexed FLIM2.8 μ mCellular resolution multiplexed FLIM tomographyzebrafish embryosmultiplexed 3 D lifetime imaging