Analysis of pit latrine microbiota reveals depth-related variation in composition, and key parameters and taxa associated with latrine fill-up rate

Posted on 2022-09-23 - 06:38

Pit latrines are used by billions of people globally, often in developing countries where they provide a low-tech and low-cost sanitation method. However, health and social problems can arise from a lack of emptying or maintenance of these facilities. A better understanding of the biological and environmental parameters within pit latrines could inform attempts to enhance material decomposition rates, and therefore slow fill-up rate. In this study, we have performed a spatial analysis of 35 Tanzanian pit latrines to identify bacteria and environmental factors that are associated with faster or slower pit latrine fill-up rates. Using ordination of microbial community data, we observed a linear gradient in terms of beta diversity with increasing pit latrine sample depth, corresponding to a shift in microbial community structure from gut-associated families in the top layer to environmental- and wastewater-associated taxa at greater depths. We also investigated the bacteria and environmental parameters associated with fill-up rates, and identified pH, volatile solids, and volatile fatty acids as features strongly positively correlated with pit latrine fill-up rates, whereas phosphate was strongly negatively correlated with fill-up rate. A number of pit latrine microbiota taxa were also correlated with fill-up rates. Using a multivariate regression, we identified the Lactobacillaceae and Incertae_Sedis_XIII taxa as particularly strongly positively and negatively correlated with fill-up rate, respectively. This study therefore increases knowledge of the microbiota within pit latrines, and identifies potentially important bacteria and environmental variables associated with fill-up rates. These new insights may be useful for future studies investigating the decomposition process within pit latrines.

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Ijaz, Umer Zeeshan; Gundogdu, Ozan; Keating, Ciara; van Eekert, Miriam; Gibson, Walter; Parkhill, Julian; et al. (2022): Analysis of pit latrine microbiota reveals depth-related variation in composition, and key parameters and taxa associated with latrine fill-up rate. Frontiers. Collection. https://doi.org/10.3389/fmicb.2022.960747
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Frontiers in Microbiology

AUTHORS (14)

Umer Zeeshan Ijaz
Ozan Gundogdu
Ciara Keating
Miriam van Eekert
Walter Gibson
Julian Parkhill
Faraji Abilahi
Benard Liseki
Viet-Anh Nguyen
Steven Sudgen
Christopher Quince
Jeroen H. J. Ensink
Belen Torondel
Alan W. Walker
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