Peter Barr-Gillespie

Publications

• ELMOD1 stimulates ARF6-GTP hydrolysis to stabilize apical structures in developing vestibular hair cells
• Heterodimeric capping protein is required for stereocilia length and width regulation
• Mass spectrometry quantitation of proteins from small pools of developing auditory and vestibular cells
• Reverse transduction measured in the living cochlea by low-coherence heterodyne interferometry
• Localization of PDZD7 to the stereocilia ankle-link associates this scaffolding protein with the usher syndrome protein network
• Na+/K+-ATPase α1 identified as an abundant protein in the blood-labyrinth barrier that plays an essential role in the barrier integrity
• Assembly of hair bundles, an amazing problem for cell biology
• The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice
• Harmonin Mutations Cause Mechanotransduction Defects in Cochlear Hair Cells
• Molecular architecture of the chick vestibular hair bundle
• Stereocilia membrane deformation: Implications for the gating spring and mechanotransduction channel
• Measurement of cochlear power gain in the sensitive gerbil ear
• Bottoms up: Transduction channels at tip link bases
• Monitoring Intracellular Calcium Ion Dynamics in Hair Cell Populations with Fluo-4 AM
• Large membrane domains in hair bundles specify spatially constricted radixin activation
• Improved Biolistic Transfection of Hair Cells
• Distinct energy metabolism of auditory and vestibular sensory epithelia revealed by quantitative mass spectrometry using MS2 intensity
• Digenic inheritance of deafness caused by 8J allele of myosin-VIIA and mutations in other Usher I genes
• Reply to "on cochlear impedances and the miscomputation of power gain" by Shera et al. J. Assoc. Re. Otolaryngol.
• Myosin 1c participates in B cell cytoskeleton rearrangements, is recruited to the immunologic synapse, and contributes to antigen presentation
• Mechanotransduction by Hair Cells: Models, Molecules, and Mechanisms
• Loss-of-function mutations of ILDR1 cause autosomal-recessive hearing impairment DFNB42
• Unraveling cadherin 23's role in development and mechanotransduction
• Twist-off purification of hair bundles
• The local forces acting on the mechanotransduction channel in hair cell stereocilia
• Accurate label-free protein quantitation with high- and low-resolution mass spectrometers
• Tip-link protein protocadherin 15 interacts with transmembrane channel-like proteins TMC1 and TMC2
• Correlation of actin crosslinker and capper expression levels with stereocilia growth phases
• Who needs tip links? Backwards transduction by hair cells
• Mechanotransduction: The elusive hair cell transduction channel revealed?
• Erratum: High-resolution structure of hair-cell tip links (Proceedings of the National Academy of Sciences of the United States of America (2000) 97 (13336-13341) DOI: 10.1073/pnas.97.24.13336)
• Molecular Remodeling of Tip Links Underlies Mechanosensory Regeneration in Auditory Hair Cells
• Probing the Cochlear Amplifier by Immobilizing Molecular Motors of Sensory Hair Cells
• Molecular Biology of Hearing and Balance
• The proteome of mouse vestibular hair bundles over development
• Hair-bundle proteomes of avian and mammalian inner-ear utricles
• PDZD7-MYO7A complex identified in enriched stereocilia membranes
• Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid
• New treatment options for hearing loss
• A short splice form of Xin-actin binding repeat containing 2 (XIRP2) lacking the xin repeats is required for maintenance of stereocilia morphology and hearing function
• Analysis of the Proteome of Hair-Cell Stereocilia by Mass Spectrometry
• Neuroplastin isoform Np55 is expressed in the stereocilia of outer hair cells and required for normal outer hair cell function
• Stereocilia-staircase spacing is influenced by myosin III motors and their cargos espin-1 and espin-like
• Twist-off purification of hair bundles
• Annexin A5 is the Most Abundant Membrane-Associated Protein in Stereocilia but is Dispensable for Hair-Bundle Development and Function
• Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt)
• Single-cell proteomics reveals downregulation of TMSB4X to drive actin release for stereocilia assembly
• Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cells
• Single-cell proteomics reveals changes in expression during hair-cell development
• Stereocilia Rootlets: Actin-Based Structures That Are Essential for Structural Stability of the Hair Bundle
• Silencing the Cochlear Amplifier by Immobilizing Prestin
• Hair bundles: Keeping it together
• Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides
• Myosin Iβ is located at tip link anchors in vestibular hair bundles
• Myosin and adaptation by hair cells
• Differential localization of unconventional myosin I and nonmuscle myosin II during B cell spreading
• Metazoan mechanotransduction mystery finally solved
• Fast adaptation in vestibular hair cells requires Myosin-1c activity
• Myosin I and adaptation of mechanical transduction by the inner ear
• The Enteropathogenic E. coli Effector EspB Facilitates Microvillus Effacing and Antiphagocytosis by Inhibiting Myosin Function
• Hair Bundles Are Specialized for ATP Delivery via Creatine Kinase
• Apical phosphatidylserine externalization in auditory hair cells
• A mechanism for active hearing
• Vestibular hair bundles control pH with (Na+, K +)/H+ exchangers NHE6 and NHE9
• Physical and functional interaction between protocadherin 15 and myosin VIIa in mechanosensory hair cells
• Na+ influx triggers bleb formation on inner hair cells
• The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function
• Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles.
• Xenopus TRPN1 (NOMPC) localizes to microtubule-based cilia in epithelial cells, including inner-ear hair cells
• Have we found the tip link, transduction channel, and gating spring of the hair cell?
• Hair-cell mechanotransduction and cochlear amplification
• Erratum: Na+ influx triggers bleb formation on inner hair cells (American Journal of Physiology - Cell Physiology (June 2005) 288 (C1332-C1341) doi:10.1152/ajpcell.0522.2004
• Myosin-1c, the hair cell's adaptation motor
• Myosin-VIIa and transduction channel tension
• Improved electrophoresis and transfer of picogram amounts of protein with hemoglobin
• Hair cells require phosphatidylinositol 4,5-bisphosphate for mechanical transduction and adaptation
• Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic
• Cadherin 23 Is a component of the tip link in hair-cell stereocilla
• Hearing aid
• Fast adaptation in the mammalian cochlea: A conserved mechanism for cochlear amplification
• The hair cell's transduction channel
• Unconventional myosins in inner-ear sensory epithelia
• Myosin-I isozymes in neonatal rodent auditory and vestibular epithelia
• A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells
• Myosin-I nomenclature
• Molecular basis of mechanosensory transduction
• Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides.
• High-resolution structure of hair-cell tip links
• Engineering of the myosin-Iβ nucleotide-binding pocket to create selective sensitivity to N6-modified ADP analogs
• Deaf and dizzy mice with mutated myosin motors
• Chemical-genetic inhibition of sensitized mutant unconventional myosins.
• Seeing touch: moving closer to the worm mechanotransduction complex.
• Developmental Assembly of Transduction Apparatus in Chick Basilar Papilla
• Myosin-1c Interacts with Hair-Cell Receptors through Its Calmodulin-Binding IQ Domains
• Plasma membrane Ca2+-ATPase isoform 2a is the PMCA of hair bundles
• Localization of myosin-Iβ near both ends of tip links in frog saccular hair cells
• Multiple myosin motors and mechanoelectrical transduction by hair cells
• Plasma membrane Ca2+-ATPase extrudes Ca2+ from hair cell stereocilia
• Feeling force: Mechanical transduction by vertebrates and invertebrates
• Multiple myosin isozymes and hair-cell function
• Regeneration of broken tip links and restoration of mechanical transduction in hair cells
• Phosphate analogs block adaptation in hair cells by inhibiting adaptation-motor force production
• Molecular machinery of auditory and vestibular transduction
• Pulling springs to tune transduction: Adaptation by hair cells
• Molecular genetic analysis of myoF, a new Dictyostelium myosin I gene
• Identification of a 120 kd hair-bundle myosin located near stereociliary tips
• Adenine nucleoside diphosphates block adaptation of mechanoelectrical transduction in hair cells
• Calmodulin and calmodulin-binding proteins in hair bundles
• Chemiluminescence detection of proteins from single cells
• High-purity isolation of bullfrog hair bundles and subcellular and topological localization of constituent proteins
• A soluble form of bovine rod photoreceptor phosphodiesterase has a novel 15-kDa subunit
• Inhibition and stimulation of photoreceptor phosphodiesterases by dipyridamole and M and B 22,948
• cGMP is tightly bound to bovine retinal rod phosphodiesterase
• Characterization of a bovine cone photoreceptor phosphodiesterase purified by cyclic GMP-Sepharose chromatography
• Control of stereocilia length during development of hair bundles
• GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region
• Spontaneous allelic variant inUsh1gresulting in an expanded phenotype
• GIPC3 couples to MYO6 and PDZ domain proteins, and shapes the hair cell apical region