manual fos box placements.pdf (32.11 MB)
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Distant Fos Counting template

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posted on 17.04.2020, 16:13 authored by Shelley WarlowShelley Warlow, Erin Naffziger

This document shows sampling regions within each Brain site that were quantified. Three 100 x 100 micron boxes were placed at arbitrary sites within pre-defined brain regions defined by Paxinos and Watson rat brain atlas (2014).

Dataset supporting: Warlow, S.M., Naffziger, E.E. & Berridge, K.C. The central amygdala recruits mesocorticolimbic circuitry for pursuit of reward or pain. Nat Commun 11, 2716 (2020). https:/

Abstract: How do brain mechanisms create maladaptive attractions? Here intense maladaptive attractions were created in laboratory rats by pairing optogenetic channelrhodopsin (ChR2) stimulation of central nucleus of amygdala (CeA) in rats with encountering either sucrose, cocaine, or a painful shock-delivering object. Pairings made the respective rats pursue either sucrose exclusively, or cocaine exclusively, or repeatedly self-inflict shocks. CeA-induced maladaptive attractions, even to the painful shock-rod, recruited mesocorticolimbic incentive-related circuitry. Shock-associated cues also gained positive incentive value and were pursued. Yet the motivational effects of paired CeA stimulation could be reversed to negative valence in a Pavlovian fear learning situation, where CeA ChR2 pairing increased defensive reactions. Finally, CeA ChR2 valence could be switched to neutral by pairing with innocuous stimuli. These results reveal valence plasticity and multiple modes for motivation via mesocorticolimbic circuitry under the control of CeA activation.

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  • DA - National Institute on Drug Abuse (NIDA)
  • MH - National Institute of Mental Health (NIMH)

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