vhs accounts for the translational shutoff zone in an advancing infection.

(A) Cells were infected with either HSV-1[17] or HSV-1[17].Δvhs according to the standard workflow in Fig 1b, and analysed (25 hr) for newly synthesised protein (green) or accumulated VP5 (red). White asterisks denote the cells in shutoff phase. (B) Cells were infected with different mutants of HSV-2[186]; ΔUL41 or its repaired strain ΔUL41-REP and D215N or its repaired strain D215N-REP and analysed as above (25 hr). White asterisks denote the cells in shutoff phase.