ANG1 treatment increases vessel maturation following treatment in mdx/utrn+/- GM muscle.

A. Representative immunofluorescence images of αSMA expression (green) in sham-injected, VEGF- and VEGF+ANG1-treated GM muscles. B. Immunohistochemical analysis of αSMA-positive vessels, represented as percent image area. n = 4 for all groups, by one-way ANOVA. Error bars represent SD and means with different letters are significantly different. C. Growth factor-coated beads are visible at the injection site. αSMA-positive vessels are detected at the injection site of VEGF+ANG1- and ANG1-treated hind limbs, and absent at the injection site of sham-injected and VEGF-treated hind limbs. DAPI was used as a counterstain. Scale bar = 100 μm.