MicroRNA-binding thermodynamics and kinetics by RNA Bind-n-Seq

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posted on 2022-02-16, 10:35 authored by Karina JouravlevaKarina Jouravleva, Joel Vega-Badillo, Phillip Zamore

RBNS was developed to qualitatively describe the specificity of RNA-binding proteins. Here, we present an analytical strategy to measure absolute affinity values and relative association and dissociation rate constants from RBNS data. All original code and two data examples have been deposited here.

Funding

NIH grant R35 GM136275

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Keywords

RNA Bind-n-Seq binding affinity dissociation constant association rate constant dissociation rate constant Biochemistry Biophysics

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CC BY 4.0

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