pone.0260650.s001.pptx (107.01 kB)
MS of sample supernatant of E. coli O113:H21 strain RM7806 cultured overnight on LBA supplemented with 400 ng/mL of MMC.
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posted on 2021-11-29, 18:39 authored by Clifton K. Fagerquist, Claire E. DoddProtein ion at m/z 9779 (marked with star) was analyzed by MS/MS (Fig 4, bottom panel).
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whole genome sequencingtype fragment ionsnearly complete concurrencediv >< pintact protein ionsresidue located withinpsd fragment ionsintramolecular disulfide bondsprotein sequences derivedfourteen proteins producedescherichia coli software correctly identifiedhighly specific ms), glutamic acidtruncated protein sequenceshost proteins producedtruncated sequencespsd ),host proteinsone proteinone residuedisulfide loopaspartic acidtwo proteinssilico nine proteinsabsence translationally modifiedterminal sideterminal methioninesource decaysignal peptideresults demonstrateobtained excepteither removalcleavage site
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