HIV-1 infection of CD4TL induces the death of bystander cells but only moderately the death of productively infected cells (Related to Fig 6).

Armani-Tourret, Marie; Zhou, Zhicheng; Gasser, Romain; Staropoli, Isabelle; Cantaloube-Ferrieu, Vincent; Benureau, Yann; Garcia-Perez, Javier; Pérez-Olmeda, Mayte; Lorin, Valérie; Puissant-Lubrano, Bénédicte; Assoumou, Lambert; Delaugerre, Constance; Lelièvre, Jean-Daniel; Lévy, Yves; Mouquet, Hugo; Martin-Blondel, Guillaume; Alcami, Jose; Arenzana-Seisdedos, Fernando; Izopet, Jacques; Colin, Philippe; Lagane, Bernard

doi:10.1371/journal.ppat.1009526.s007

ppat.1009526.s007.pptx (493.49 kB)

HIV-1 infection of CD4TL induces the death of bystander cells but only moderately the death of productively infected cells (Related to Fig 6).

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posted on 2021-04-19, 17:34 authored by Marie Armani-Tourret, Zhicheng Zhou, Romain Gasser, Isabelle Staropoli, Vincent Cantaloube-Ferrieu, Yann Benureau, Javier Garcia-Perez, Mayte Pérez-Olmeda, Valérie Lorin, Bénédicte Puissant-Lubrano, Lambert Assoumou, Constance Delaugerre, Jean-Daniel Lelièvre, Yves Lévy, Hugo Mouquet, Guillaume Martin-Blondel, Jose Alcami, Fernando Arenzana-Seisdedos, Jacques Izopet, Philippe Colin, Bernard Lagane

PHA/IL-2-treated CD4TL (2 x 10⁵ cells) were incubated with 50 ng p24 of SENS or RES viruses for 7 days. Productively infected (p24 positive) and bystander (p24 negative) CD4TL were then stained with the Live/Dead viability dye (L/D) and analyzed by flow cytometry. A typical experiment is shown. We measured about 10% of L/D positive cells in uninfected CD4TL cells.

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