Additional file 3: of Characterization of sulfhydryl oxidase from Aspergillus tubingensis

AtSOX activity measured by HVA-peroxidase coupled assay using reduced GSH (5 mM) as a substrate according to [33]. The enzyme reaction is at the enzymatic rate (linear area ca. 0 - 150 s). The production of the fluorescent HVA dimer was followed at excitation wavelength 320 nm and emission wavelength 420 nm. The reduced AtSOX activity with the inhibitor zinc sulphate (10 mM) is also shown (dashed line). The triplicate measurements were done. (PPTX 121 kb)