Additional file 2 of Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma
posted on 2021-01-19, 04:39authored byAurore Dumond, Etienne Brachet, Jérôme Durivault, Valérie Vial, Anna K. Puszko, Yves Lepelletier, Christopher Montemagno, Marina Pagnuzzi-Boncompagni, Olivier Hermine, Christiane Garbay, Nathalie Lagarde, Matthieu Montes, Luc Demange, Renaud Grépin, Gilles Pagès
Additional file 2: Fig. S1. Study of down-regulation of NRPs by shRNA in 786-O cells. (A-B) Effects of the downregulation of NRP by shRNA on NRP1 and NRP2 mRNA expression measured by qPCR. (C) Effects on cell metabolic activity measured by MTT assays. (D) Down-regulation of NRPs decreased cell migration. Bevacizumab increased this effect for NRP1 down-regulation. (E) Down-regulation of NRPs had no effect on VEGFA and VEGFC production measured by ELISA. *p < 0.05; **p < 0.01; *** p < 0.001.
Funding
Helsinn Centre Scientifique de Monaco Fondation de France Institut National Du Cancer Agence Nationale Recherche (ANR) Ligue Contre le Cancer Conseil général des Alpes Maritimes Association Cordons de Vie Fondation François Xavier Mora Fondation Flavien