Additional file 2 of Integrated NIRS and QTL assays reveal minor mannose and galactose as contrast lignocellulose factors for biomass enzymatic saccharification in rice

Additional file 2: Figure S1. Applicability test of previously established NIRS modes [35]. A Spectrum variation range of prediction set and modeling set. B Distribution of prediction set and modeling set in principal components space of spectra. C–E Accuracy verification of NIRS models by comparing experimental and predicted values of 20 rice samples. ASL, acid soluble lignin; AIL, acid insoluble lignin; Glc-Rel1, glucose released after biomass enzymatic digestion following 1% (m/v) NaOH pretreatment. The fitting equations and coefficients are shown at the top of the plot. * and ** as significant correlations at P < 0.05 and 0.01, respectively. Figure S2. Frequency distributions of monosaccharides, acid soluble/insoluble lignin and biomass saccharification. A Mannose; B Galactose; C Acid soluble lignin; D Acid insoluble lignin; E Glucose yields released from enzymatic hydrolyses after 1% NaOH pretreatments (n = 215). Figure S3. Correlation analysis between lignin/cellulose related traits and enzymatic saccharification of alkaline pretreated rice straw. (A–G) Correlations of ρ-hydroxy-phenyl lignin H, syringyl lignin S, guaiacyl lignin G, acid soluble lignin (ASL), acid insoluble lignin (AIL), cellulose crystallinity index (CrI) and crystalline cellulose (Cry-cel) with glucose released after enzymatic digestion following 0.025% (m/v) NaOH pretreatment (Glc-Rel0.025). (H–N) Correlations of H, S, G, ASL, AIL, CrI and Cry-cel with glucose released after enzymatic digestion following 1% (m/v) NaOH pretreatment (Glc-Rel1). The fitting equations and coefficients are shown at the top of the plot. * and ** indicate the correlations are significant at p < 0.05 and 0.01, respectively. n = 100