12935_2021_1765_MOESM1_ESM.pptx (10.76 MB)
Additional file 1 of Postponing tumor onset and tumor progression can be achieved by alteration of local tumor immunity
presentation
posted on 2021-04-16, 10:38 authored by Yan Mei, Mingdian Wang, Guanming Lu, Jiangchao Li, Lixia Peng, Yanhong Lang, Mingming Yang, Lingbi Jiang, Changzhi Li, Lisheng Zheng, Zhijie Liu, Dehuan Xie, Lingling Guo, Bijun Huang, Musheng Zeng, Yanxia Shi, Chaonan QianAdditional file 1: Fig. S1. The experiment flow chart of this study was depicted. Fvb.B6 F1 female (brown color) hybrids were generated by crossing FVB/N-Tg(MMTV-PyMT)634Mul male (white color) mice to C57BL/6J female (black color) mice (Fig. S1A), Mammary tumors were harvested when the diameter of the largest tumor per mouse reached approximately 2 millimeters and 10 millimeters, which correspond to the Adenoma/MIN and late carcinoma stages. Lung metastases were harvested at the age of 13 weeks for Fvb mice and 16 weeks for F1 hybrids (Fig. S1B).
