DPMAS: pipeline for 450K top table replication with MiSeq amplicons

DNA methylation is one of the most important epigenetic modifications in gene expression regulation and it plays a crucial role in multiple biological processes. Therefore, DNA methylation profiling is important to reveal regions of the genome that are altered during development or that are perturbed by disease. Methylation signals at CpG sites can be assessed using bisulfite conversion method and massive sequencing technologies. Here we present the implementation of a bioinformatics pipeline for replication with MiSeq sequencing amplicons of the top hits obtained from Infinium 450K differential methylation experiments.