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Metadata record for the manuscript: TERT promoter hotspot mutations and gene amplification in metaplastic breast cancer
Summary
This metadata record provides details of the data supporting the claims of the related manuscript: “TERT promoter hotspot mutations and gene amplification in metaplastic breast cancer”.
The related study sought to determine the frequency of genetic alterations affecting TERT in a cohort of 60 metaplastic breast cancers (MBCs) with distinct predominant metaplastic components (squamous, 23%; spindle, 27%; osseous, 8%; chondroid, 42%), and to compare the repertoire of genetic alterations of MBCs according to the presence of TERT promoter hotspot mutations or gene amplification.
Type of data: whole exome sequencing, histologic images
Subject of data: Homo sapiens
Sample size: 60 primary MBCs
Population characteristics: Archived tumours classified as metaplastic breast cancer with representative materials available were selected for this study and retrospectively analysed.
Data access
The whole-exome sequencing data supporting Figures 2, 3, Supplementary Figures S2 and S3, and Supplementary Tables S1, S2 and S3 are openly available in the Sequence Read Archive via the following accession: https://identifiers.org/ncbi/insdc.sra:SRP073692. These data were first described in the original publication by Ng et al (https://doi.org/10.1158/1078-0432.CCR-16-2857).
MSK-IMPACT sequencing data of 3 samples included in the MSK-IMPACT Clinical Sequencing Cohort supporting Figures 2, 3, Supplementary Figures S2, S3, and Supplementary Tables S1 and S2 are publicly available at cBioPortal (https://identifiers.org/cbioportal:msk_impact_2017). These data were first described in the original publication by Zehir et al (https://doi.org/10.1038/nm.4333).
Sequencing data of 19 previously unreported MBCs (5 subjected to whole-exome sequencing and 14 to MSK-IMPACT sequencing) are available at cBioPortal (https://identifiers.org/cbioportal:mbc_msk_2021).
Additionally, the following data are available upon request from the corresponding authors: Histologic images supporting Figure 1 and Supplementary Figure S1; Sanger sequencing electropherograms supporting Figure 2, Table 2, and Supplementary Figures S1 and S4; Clinicopathologic data supporting Figure 2 and 3, Table 1, and Supplementary Table S1.
Corresponding author(s) for this study
Dr. Jorge S. Reis-Filho, MD PhD FRCPath, Department of Pathology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA. Email: reisfilj@mskcc.org.
Dr. Hong Zhang, MD, Department of Pathology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA. Email: zhangh3@mskcc.org.
Study approval
Institutional Review Board (IRB) of Memorial Sloan Kettering Cancer Center (MSKCC)
