pone.0166940.g003.tif (668.61 kB)

miR-125b negatively regulated SGPL1 in trophoblast cells.

figure

posted on 2016-12-09, 18:56 authored by Weiwei Yang, Anning Wang, Chunling Zhao, Qinghua Li, Zhifang Pan, Xuefu Han, Cuijuan Zhang, Guohui Wang, Chao Ji, Guili Wang, Guangtao Jia, Jiyu Ju, Wei Gao, Wenjing Yu, Xiaoying Liu, Xi Chen, Weiguo Feng, Zhiqin Gao, Jie Li, Chune Ren

(A) The expression of miR-125b in HTR8/SVneo cells transfected with miR-125b mimics (miR-125b), scramble control (NC) or mock was revealed by Real-time qPCR. (B) The expression of miR-125b in HTR8/SVneo cells transfected with miR-125b inhibitor, scramble inhibitor (NC inhibitor) or mock was revealed by Real-time qPCR. Relative expression of miR-125b was normalized by U6. Both mRNA (C) and protein (E) levels of SGPL1 in HTR8/SVneo cells transfected with scramble control (NC) and miR-125b mimics (miR-125b) were revealed by Real-time qPCR and western blot. Both mRNA (D) and protein (F) levels of SGPL1 in HTR8/SVneo cells transfected with miR-125b inhibitor (miR-125b inhibitor) and scramble control inhibitor (NC inhibitor) were revealed by Real-time qPCR and Western blot. All experiments were repeated ≥3 times independently in identical conditions. Results are presented as mean ± SEM. Statistical comparison in separate groups between miR-210 and NC was performed using SPSS, with p<0.05 considered as significant. *p < 0.05, **p < 0.01.