GUV.mp4

A slowed-down video of a fluctuating POPC vesicle containing 40 mol% DOPE (same vesicle as in Figure 1 in the main text). The vesicle was prepared in 20 mM sucrose, 4-fold diluted in 22 mM glucose, and additionally deflated by leaving the observation chamber open for 5 minutes. The sequence shows phase contrast images acquired at 100 frames per second and displayed at 25 frames per second (processing was done with Fiji). The approximate duration is roughly 4 seconds in real time (time stamps shown in the upper left corner).