An embryological twist in zebra fish embryo, as revealed by cellular movement patterns.

Source

These videos belong to the appendix of de Lussanet & Osse (2012). The publisher (Brill, Leiden, NL) no longer provides a public link. The copyright rests with the authors.

Appendix A.1. Additional movie 1: low.mov

Supplementary movie A.1 is based on the supplementary movie S2 of Keller et al. (2008), (with permission) © Keller www.embl.de/digitalembryo. For this we extracted a number of movie frames from their supplementary Movie S2. During this period (865-1000 min) the cells that will form the prospective eye cell masses, migrate clockwise (encircled blue and red respectively), whereas the future mid- and hindbrain rotate anti-clockwise (green arrows). The prospective right eye cell mass (blue) is initially invis- ible because it is hidden by the embryo. Starting from 865 min, every 15-min a frame was marked. For accurate play- back, please select “Play All Frames” in the QuickTime Player. Note that the beginning and end of the movie are sped-up.

Appendix A.2. Additional movie 2: selected.mov

Movie A.2 is a sped-up version of Additional movie 1, to show more clearly the axial compensatory movements. Advice for repeated playing: select “Loop” in your Quick- time player.

References

de Lussanet, Marc H. E. and Osse, Jan W. M. (2012) An ancestral axial twist explains the contralateral forebrain and the optic chiasm in vertebrates. Animal Biol., 62(2):193–216. doi: 10.1163/157075611X617102. URL http://arxiv.org/abs/1003.1872.

Philipp J. Keller, Annette D. Schmidt, Joachim Wittbrodt, and Ernst H. K. Stelzer. Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy. Science, 322(5904):1065–1069, 2008. doi: 10.1126/sci- ence.1162493.