bZIP domain of BIP1.

A. Alignment of bZIP domains from BIP1, BIP1 orthologues from N. crassa (NcBIP1, NCU03847), F. graminearum (FgBIP1, FGRAMPH1_01G06311), B. cinerea (BcBIP1, Bcin09g05210), A. nidulans (AnBIP1, ANIA_00825) and P. nodorum (SNOG_11592). S. cerevisiae Gcn4 (ScGCN4, YEL009C) and S. cerevisiae Yap1 (ScYAP1, YML007W) TFs were added for comparison. bZIP domains were extracted from protein sequences and aligned using Clustal omega. 100% identical amino acids are highlighted in black. 90–80% similar amino acids are highlighted in dark grey. 70–60% similar amino acids are highlighted in light grey. NLS: nuclear localization signal predicted using Hidden Markov Model for nuclear localization signal prediction. B. Functional domains identified in BIP1 protein using CDD database (bZIP-YAP, CD14688 domain) and previous analysis using YAP1 fungal TFs (A).

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