CYP26A1/B1/C1 expression during fetal retinal development and organoid differentiation experiments.

(A–C) Expression of CYP26A1, CYP26B1, and CYP26C1 in fetal human retinas by day of gestation and retinal region. CPM, log counts per million. Analyzed from [16]. Error bars for the 2 samples from fetal day 94 indicate SEM. Original data sets are in S3 Data. (A) Whole retina. (B) Central retina. (C) Periphery. (D) Protocol for human retinal organoid differentiation, adapted from [15]. (E) Expression of THRB (cone marker) and NRL (rod marker) during retinal organoid development. TPM, transcripts per million. Analyzed from [15]. Original data sets are in S6 Data. (F) No significant differences in overall densities of M + L cones at day 200 in early RA treatment conditions (as in Fig 3F–3H) (Dunnett’s multiple comparison’s test, against “No RA” control: “RA to day 60” p = 0.98, “RA to day 130” p = 0.32). Significant difference between “No RA” and “Late RA” conditions (Dunnett’s multiple comparison’s test, * indicates p < 0.05) (as in Fig 3I). Error bars indicate SEM. Individual circles represent individual organoids. Original data sets are in S3 Data. (G) Representative brightfield image of a retinal organoid in “RA to day 130” conditions. (H) Representative brightfield image of a retinal organoid in “Late RA” conditions.

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