Western blot of SPO11, CAS9, and eGFP in adult testis.

(A–C) Western blot of (A) SPO11, (B) CAS9, and (C) eGFP on 3 wild-type and 3 Spo11^{Cas9-P2A-eGFP/+} adult testes. (A’–C’) Serially-stained membranes adding anti β-ACTIN to detect the loading control. PP, Precision Plus Ladder; MM, MagicMark Ladder. The raw gel images can be found at the associated Zenodo data repository (https://doi.org/10.5281/zenodo.5510697) in the file labeled “S1 Raw Images.pdf.”

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