posted on 2021-10-20, 17:35authored byLuisa Vernizzi, Christian F. Lehner
Spermatocytes expressing His2Av-mRFP and Cenp-A/Cid-EGFP were analyzed by time-lapse imaging. The number of centromeric Cid-EGFP dots was determined and plotted over time. The graphs from a subset of the analyzed spermatocytes are displayed for illustration of the considerable temporal variability of centromere de-clustering. t = 0 was set at the transition from the three- to the four-dot stage.