pone.0287829.s002.pdf (75.96 kB)
Validation of interferon activation upon siRNA transfection into iDC.
journal contribution
posted on 2023-11-01, 17:38 authored by Tomozumi Imamichi, Qian Chen, Bharatwaj Sowrirajan, Jun Yang, Sylvain Laverdure, Mayra Marquez, Anthony R. Mele, Catherine Watkins, Joseph W. Adelsberger, Jeanette Higgins, Hongyan SuiMonocytes (1x107 cells) were transfected with si-Ctrl or si-ANKRD22 using HiPerFect (Qiagen) as described in the Materials and Methods. As a control, monocytes were mock treated with HiPerFect alone. The transfected cells were cultured for 7 days in G4-media. The cells were collected for RNA extraction on day-4 and day-7. Relative IFN-α8, IFN-α14, IFN-β, or IFN-λ1 expression was measured by qRT-PCR. two independent assay was performed, and one of the representative dates is shown. Data are shown as mean ± SD (n = 3), and the gene expression level was compared to mock-treated cells. ** p,0.01, ***p< 0.001.
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