pbio.3002544.s006.pdf (944.47 kB)
Unanchored K63-linked Ub chains enhance VP35 –NP interactions.
journal contribution
posted on 2024-02-29, 18:53 authored by Carlos A. Rodríguez-Salazar, Sarah van Tol, Olivier Mailhot, Maria Gonzalez-Orozco, Gabriel T. Galdino, Abbey N. Warren, Natalia Teruel, Padmanava Behera, Kazi Sabrina Afreen, Lihong Zhang, Terry L. Juelich, Jennifer K. Smith, María Inés Zylber, Alexander N. Freiberg, Rafael J. Najmanovich, Maria I. Giraldo, Ricardo Rajsbaum(A) The addition of unanchored K63-linked polyUb chains enhances WT VP35 interactions with NP. Lysates from HEK293T cells expressing WT-VP35 were mixed with lysate from cells expressing NP, in the presence or absence of added purified unanchored K63-linked polyUb chains [2–12], followed by coIP with anti-Flag beads. (B) Treatment with pCEBS or SFC in a minigenome assay reduces interactions between VP35 and NP in the co-immunoprecipitation assay. HEK293T cells were transfected with minigenome components and 4 h post-transfection cells were treated with 200 μm of pCEBS and SFC; 50 h later cells were lysed, and immunoprecipitation assay was performed using FLAG beads.
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