UNRAVELING THE POTENTIAL PHYTOCHEMICAL COMPOUNDS OF GYMNEMA SYLVESTRE THROUGH GC-MS STUDY

ABSTRACT

Objective: To profile the chemical composition of ethanolic extract of Gymnema sylvestre through Gas Chromatography-Mass Spectrometry technique.

Methods: The chemical compositions of the plant leaf extracts of G. sylvestre were investigated using Gas Chromatography–Mass Spectroscopy (Scion 436-GC Bruker model coupled with a Triple, quadruple mass spectrophotometer) and NIST-MS library.

Results: GC-MS analysis of leaf extracts revealed the existence of Terpenes, alcohols, fatty acids, amine and sterols. The highest % Peak area is hexadecanoic acid, α-Santoline alcohol, recorded the next highest % peak area of 9.05. Major of the compounds belongs to terpeneoid group, namely 6-Octen-1-ol, 3,7-dimethyl, Isophytol, Squalene, Nerolidol, β-Amyrin and Cedrene-V6 which constitutes 30.7% of the peak area. The presence of α-Tocopherol-β-D-mannoside and Vitamin E also identified through this study.

Conclusion: From the above finding we can interpret that the G. sylvestre contained a considerable amount of phytoconstituents especially terpenoids. In future, this study will be helpful for the quantitative analysis of phytochemicals as well as formulation studies.