pgen.1009725.s003.pdf (325.36 kB)
Two mutant alleles in tocopherol cyclase (Cre01.g013801, VTE1) in ARC.
journal contribution
posted on 2021-09-07, 17:47 authored by Setsuko Wakao, Patrick M. Shih, Katharine Guan, Wendy Schackwitz, Joshua Ye, Dhruv Patel, Robert M. Shih, Rachel M. Dent, Mansi Chovatia, Aditi Sharma, Joel Martin, Chia-Lin Wei, Krishna K. NiyogiSchematic representation of the disruption sites in CAL014_01_19, a strictly acetate-requiring mutant and CAL032_02_19, a mutant with comparatively moderate phenotype.
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systematic manual analysispreviously unknown proteinsphylogenomically defined setgreatly advanced effortsdiv >< ppreviously characterized functionsone end alignedscale mutant librariesprotein functional analysisconfidence candidate genesadvancing gene discovery>, land plantschlamydomonas reinhardtii plasmid insertion siteschlamydomonas plasmid insertionfunctions unrelatedend sequencesmutant librarymutant generatedfunctional annotationsfunctional annotationcrpsbp4 crlpa3 tetrapyrrole synthesisresults connectresulting higherrequiring mutantsphotoautotrophic growthmolecular extentlinearized plasmidinsertional mutagenesisgreen algaegenomic dnagenome sequencinggenome sequencesgenetic studiesdisrupted genesconserved genescomplex rearrangementschromosomal locationchromosomal lesionsanalyze mutants96 %)509 mutants253 higher1470 genes
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