posted on 2024-01-16, 14:47authored byXueshan Bai, Renxian Wang, Xiaohua Hu, Qiang Dai, Jianxun Guo, Tongyu Cao, Weili Du, Yuning Cheng, Songxia Xia, Dingding Wang, Liya Yang, Li Teng, Dafu Chen, Yajun Liu
Large full-thickness skin lesions have been one of the
most challenging
clinical problems in plastic surgery repair and reconstruction. To
achieve in situ skin regeneration and perfect clinical outcomes, we
must address two significant obstacles: angiogenesis deficiency and
inflammatory dysfunction. Recently, black phosphorus has shown great
promise in wound healing. However, few studies have explored the bio-effects
of BP to promote in situ skin regeneration based on its nanoproperties.
Here, to investigate whether black phosphorus nanosheets have positive
bio-effects on in situ skin repair, we verified black phosphorus nanosheets’
positive effects on angiogenic and anti-inflammatory abilities in
vitro. Next, the in vivo evaluation performed on the rat large full-thickness
excisional wound splinting model more comprehensively showed that
the positive bio-effects of black phosphorus nanosheets are multilevel
in wound healing, which can effectively enhance anti-inflammatory
ability, angiogenesis, collagen deposition, and skin re-epithelialization.
Then, multiomics analysis was performed to explore further the mechanism
of black phosphorus nanosheets’ regulation of endothelial cells
in depth. Molecular mechanistically, black phosphorus nanosheets activated
the JAK-STAT-OAS signaling pathway to promote cellular function and
mitochondrial energy metabolism in endothelial cells. This study can
provide a theoretical basis for applying two-dimensional black phosphorus
nanosheets as nanomedicine to achieve in situ tissue regeneration
in complex human pathological microenvironments, guiding the subsequent
optimization of black phosphorus.