S3 Fig -

Throughout this figure: Error bars = S.E.M. N = numbers of independent biological replicates. Unless specified, unpaired nonparametric t-tests were used to assess the significance. *p≤ 0.05, **p≤0.01, ***p≤0.001 and ****p≤0.0001. (A) Quantification of muscle LD size in worms fed RD or HFrD. Each data point represents the measurement of random LDs in the images. ≥10 worms were measured in each independent biological replicate. N = 3. (B) Quantification of hypodermis LD size in worms fed RD or HFrD. Each data point represents the measurement of a random LD in the images. ≥10 worms were measured in each independent biological replicate. N = 3. (C) Body fat content of worms treated with RNAi against all of the DIO suppressors observed in the primary screen. RNAi was initiated from the L1 stage. pho-1 and Y71H10B.1 (Blue font) are the 2 DIO suppressors that cause leanness without developmental delay. (D) Representative images of the body fat content and distribution in worms fed HFrD from the L1 stage but treated with RNAi against rpt-5 and hsp-4 from the L4 stage. No change in fat content was observed in this condition. N = 3. (E) Representative images of the body fat content and distribution in worms treated with RNAi against pho-1 from the L4 stage. No change in fat content was observed. N = 3

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